《植物生理学报》 2011, 47(5): 521-524

植物生理学实验中一类偶氮化合物的分光光度检测

刘少霞¹, 陈贵¹, 秦萍¹, 姚远^2,*

沈阳农业大学1生物科学技术学院, 2科技开发公司, 沈阳110866

通信作者：姚远；E-mail: yaoyuanjane9296@sina.com；Tel: 024-88487093

摘　要：

偶氮化合物在可见光特定波长下有最大吸收峰, 但对这一特定波长认定目前仍不统一。本试验利用分光光度计法测 定了偶氮化合物的特定吸收峰。结果表明: NO -、对氨基苯磺酸(或磺胺)及α-萘胺在酸性条件下形成偶氮化合物特定吸收 峰在A520 nm处, 并非A510 nm、A530 nm或A540 nm。

关键词：偶氮化合物; 吸光度; 磺胺; 对氨基苯磺酸; α-萘胺

收稿：2011-01-17   修定：2011-03-16

Detection of a Kind of Azo Compound in Plant Physiology Experiment by Spectrophotometer

LIU Shao-Xia¹, CHEN Gui¹, QIN Ping¹, YAO Yuan^2,*

¹College of Biological Sciences and Biotechnology, ²Company of Sciences Technique and Development, Shenyang Agricultural University, Shenyang 110866, China

Corresponding author: YAO Yuan; E-mail: yaoyuanjane9296@sina.com; Tel: 024-88487093

Abstract:

It has been known that the absorption peak of azo compound will be observed under specified wavelength of visible light. However, the specified wavelength is inconsistent in different references to data. The visible absorption peak of azo compound was determined by spectrophotometry in this paper. The azo compound was generated by using the combination of naphthylamine with aminobenzene sulfonic acid, or sulfanilamide. The result shows that the absorption peak of azo compound is in A520 nm, rather than in A510 nm, A530 nm or A540 nm which are often used in the references.

Key words: azo compound; absorption; sulfanilamide; aminobenzene sulfonic acid; naphthylamine